Methylphenidate refers to a CNS stimulant mainly used in the treatment of ADHD (attention deficit hyperactivity disorder) in children between the age of 6 to 12, adults up to the age of 65, and adolescents. It can also be used in the treatment of narcolepsy.
However, the application of the drug may also bring some side-effects with it. Some of the common side-effects related to medicine are decreased appetite, headache, hallucinations, sweating, fever, dizziness, increased heart rate, weight loss, and others.
In the given case, due to the application of the drug, the child is having hallucinations. Thus, in the given case, the nurse should recommend the client to report the manifestations as quick as possible, and discontinue the use of medicine.
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The difference is that thymine is replaced by Uralic in mRNA.
Answer:
-use PCR to amplify the gene for GFP
-perform a restriction digestion of the GFP gene and the plasmid
-ligate together the GFP gene and the plasmid to generate a recombinant plasmid
-transform bacteria with the recombinant plasmid using electroporation
-plate the bacterial cells, and screen for positive transformants
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