Answer:
A.) Intended dilution factor : 0.01
B.) Actual dilution factor: 0.039
Explanation:
Dilution factor(DF) is ratio of the volume of the undiluted sample to total volume of diluted solution
A.) What was your intended dilution factor
This would be the the addition 1.0ml from 4.0ml to 99ml of sterile
Volume of sterile diluent = 99ml
Volume of Undiluted sample = 1.0ml
Total volume of diluted solution = 99ml + 1m = 100ml
Dilution factor = Volume of undiluted sample ÷ Total volume of diluted solution
Dilution factor = 1:100
= 1ml ÷ 100ml
= 0.01
B.) What was your actual dilution factor?
This would be the entire addition of 4.0ml to 99ml sterile diluent
Volume of sterile diluent = 99ml
Volume of Undiluted sample = 4.0ml
Total volume of diluted solution = 99ml + 4.0ml = 103ml
Dilution factor = Volume of undiluted sample ÷ Total volume of diluted solution
Dilution factor
= 4:103
= 4ml ÷ 103ml
= 0.039
