DNA extraction from living cell
DNA is present inside nucleus surrounded by nuclear membrane. then cell is surrounded by plasma membrane, it means that DNA inside living cell is enclosed in two layers.
Removal of cell membrane
In order to remove cell membrane some detergents are applied followed by physical lysis through vertex.when the first cell membrane is pierced it is removed through centifugation by adding phenol and chloroform.
Removal of nuclear membrane
Proteinase enzyme is added which help in rupturing of nuclear membrane and removal of histone protein from DNA. Then RNAse is added to remove RNA from DNA.
<span>They are not seen in nature because they are always combined with something to make something else.</span>
Answer:
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Explanation:
Exothermic is when energy is released
endothermic is when its being absorbed<span />