Answer:
a. At pH 9, the product, p-nitrophenol, will be ionized, the solution will appear yellow in color, and thus can be monitored at the wavelength of maximum absorption for the phenolate ion which is 400nm
Explanation:
In alkaline phosphatase assay, the hydrolysis of p nitrophenyl phosphate to p nitrophenol happens. When the ph is 9, the product which is p nitrophenol would undergo ionization. The solution is going to appear to be of yellow and it can be monitored at a wavelength for maximum absorption of phenolate ions at 400nm.
Option A is the answer to the question.
Stoichiometry is “quantitative relationship” among the “reactants” and the “products” in a “chemical reaction”.
<u>Explanation</u>:
In stoichiometry “stoicheion” means element and “metron” means measure in Greek. The stoichiometric calculation depends upon “stoichiometric coefficients” in a “chemical equation” which can be explained as the “number of moles” of each substance (reactants or products). Stoichiometric calculation is done as follows:
For example reaction between nitrogen and hydrogen to form ammonia as

Here stoichiometric coefficients show that “one molecule of nitrogen” reacts with “three molecules of hydrogen” to form “two molecules of ammonia”. Multiplying Avogadro number
to no of molecules in equation:


Taking molar masses into consideration:



Hence balanced equation gives stoichiometric coefficients which gives proportion by moles.
I believe it means that it takes a lot of heat in joules to make silicon vaporize so it stays solid until a great deal of heat has been added. By comparison, water has a latent heat of about 2260 joules per gram so vaporizes much more readily than silicon.
In general, we have this rate law express.:
![\mathrm{Rate} = k \cdot [A]^x [B]^y](https://tex.z-dn.net/?f=%5Cmathrm%7BRate%7D%20%3D%20k%20%5Ccdot%20%5BA%5D%5Ex%20%5BB%5D%5Ey)
we need to find x and y
ignore the given overall chemical reaction equation as we only preduct rate law from mechanism (not given to us).
then we go to compare two experiments in which only one concentration is changed
compare experiments 1 and 4 to find the effect of changing [B]
divide the larger [B] (experiment 4) by the smaller [B] (experiment 1) and call it Δ[B]
Δ[B]= 0.3 / 0.1 = 3
now divide experiment 4 by experient 1 for the given reaction rates, calling it ΔRate:
ΔRate = 1.7 × 10⁻⁵ / 5.5 × 10⁻⁶ = 34/11 = 3.090909...
solve for y in the equation
![\Delta \mathrm{Rate} = \Delta [B]^y](https://tex.z-dn.net/?f=%5CDelta%20%5Cmathrm%7BRate%7D%20%3D%20%5CDelta%20%5BB%5D%5Ey)

To this point,
![\mathrm{Rate} = k \cdot [A]^x [B]^1](https://tex.z-dn.net/?f=%5Cmathrm%7BRate%7D%20%3D%20k%20%5Ccdot%20%5BA%5D%5Ex%20%5BB%5D%5E1%20)
do the same to find x.
choose two experiments in which only the concentration of B is unchanged:
Dividing experiment 3 by experiment 2:
Δ[A] = 0.4 / 0.2 = 2
ΔRate = 8.8 × 10⁻⁵ / 2.2 × 10⁻⁵ = 4
solve for x for
![\Delta \mathrm{Rate} = \Delta [A]^x](https://tex.z-dn.net/?f=%5CDelta%20%5Cmathrm%7BRate%7D%20%3D%20%5CDelta%20%5BA%5D%5Ex)

the rate law is
Rate = k·[A]²[B]