Answer:
N-terminal Phe, C-terminal Ser
Explanation:
Amino acids connect like
NH2 -CH(R1) -CO -NH-CH(R2)-CO-.....-NH-CH(Rn)COOH
So, 1st amino acid is N -terminal , and it is Phe.
Last amino acid is C- terminal, and it is Ser.
The final step in a typical titration, that is here an acid base one would be to finally find the concentration of your unknown substance whether that be the acid or the base. The other steps are used before this to come to the correct calculation and conclusion.
Buffer solution resist the change in pH upon addition of small amount of strong acid or strong base.
Buffer consists of weak acid as HF / and its conjugate base NaF
When strong acid as HCl is added to buffer, it respond with its conjugate base to convert the strong acid to weak acid like this:
HCl (S.A) + NaF → NaCl + HF (W.A)
moles of HF we already have = M * V(in liters)
= 0.0955 M * 0.033 L = 3.15 x 10⁻³ mole
moles of HCl added = 8.00 x 10⁻⁵ mole
one mole HCl reacts with 1 mole NaF to give 1 mole HF
so the amount added to HF = 8.00 x 10⁻⁵
Total moles of HF present = (3.15 x 10⁻³) + (8.00 x 10⁻⁵) = 3.23 x 10⁻³ mole