For this question, lets apply Avagadro's law
when Pressure and temperature are constant, the volume occupied is directly proportional to the number of moles of gases.

where V-volume, n-number of moles and k - constant
Therefore at 2 instances

where V1 and n1 are for 1st instance
and V2 and n2 are for 2nd instance
therefore

V1 = 2.4 L
n1 = 3.7 mol
n2 = 3.7 + 1.6 = 5.3 mol
since more He moles are added at the 2nd instance its the sum of the moles.
V2 needs to be calculated

V2 = 2.4 x 5.3 / 3.7
= 3.4 L
Answer is 1st option 3.4 L
I looked at the question and there isn't a lot of information. im assuming the answer is carbon dioxide. its the only substance that would make sense I hope it helps.
Answer:
the plant population over time has decreased. this could make all other organisms above the plant population numbers decrease as well.
Explanation: