How much DNA can be extracted from an onion
1 answer:
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Answer:
To prepare 20,0 mL of the liquid mixture you should mix 15,3 mL of CHCl₃ with 4,7 mL of CHBr₃
Explanation:
Here you have two variables: The volume of both CHCl₃ (X) and CHBr₃ (Y). To find these two variables you must have, at least, two equations.
You know total volume is 20,0 mL. Thus:
X + Y = 20,0 mL (1)
The other equation is:
× 1,492 g/mL +
× 2,890 g/mL = 1,82 g/mL (2)
If you replace (1) in (2):
× 1,492 g/mL +
× 2,890 g/mL = 1,82 g/mL
Solving:
X = 15,3 mL
Thus, using (1):
20,0 mL - 15,3 mL = Y = 4,7 mL
Thus, to prepare 20,0 mL of the liquid mixture you must mix 15,3 mL of CHCl₃ with 4,7 mL of CHBr₃.
I hope it helps!
Explanation:
According to the given data, the turnover number can be calculated as follows.
Turnover number =
Therefore, moles of hydrolyzed is as follows.
Moles of hydrolyzed =
=
= 0.00682 moles
Now, moles of hydolyzed per second is calculated as follows.
Moles of hydolyzed per second =
=
And,
Moles of enzyme =
=
= moles
Therefore, the value of is as follows.
= per second
= per minute
= per minute
Thus, we can conclude that the turnover number () of carbonic anhydrase (in units of
) is
per minute.