Latitude or distance from the equator – Temperatures drop the further an area is from the equator due to the curvature of the earth. ... As a result, more energy is lost and temperatures are cooler.
The basic tool for determining toxicity of substances to marine and aquatic organisms is the toxicity test. In its simplest form, toxicity testing is taking healthy organisms from a container of clean water and placing into one containing the same water with a known concentration of a pollutant.