Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
A
Explanation:
Germanium and Carbon are in the same 14th group.
Atomic number refers to the proton number of the atom itself. Number of electrons in an atom (an atom that is not reacted with any other molecules / Just the atom alone), is the same as the number of protons, because each electron has 1 negative charge, and each proton 1 positive charge, where they cancel out on each other to become a neutral charge.
So, when atomic number is 6, proton number is also 6, and number of electrons will also be 6 in that atom.
Hope this helps! :)
Answer:
............................................................................
Explanation:
Answer:
Heat required = 13,325 calories or 55.75 KJ.
Explanation:
To convert a water to steam at 100 degree celsius to vapor, we have to give latent heat of vaporization to water
Which equals ,
Q = mL,
Where, m is the mass of water present
L = specific latent heat of vaporization
Here , m= 25 gram
L equals to 533 calories (or 2230 Joules)
So, Q = 25×533 = 13,325 Calories
Or , Q = 55,750 Joules = 55.75 KJ
so, Heat required = 13,325 calories or 55.75 KJ.
