Answer:
Enzyme-linked Immunosorbent Assay, also know as ELISA are used to determine if an antibody is present in a patient's blood sample. They are found in bodily fluids.
Explanation:
The three important limitations are:
1) A positive result confirming a presence of an antibody but it not necessarily making the patient sick
2) A false negative where the amount of antibodies is too low to be measured
3) Another positive result may occur if an unrelated antibody reacts with the antigen non specifically.
