Based on the dilution formula, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.
<h3>How can 50-fold dilution of the enzyme be done?</h3>
The 50-fold dilution of the stock enzyme solution can be done by using the dilution formula to determine the given volume of the stock solution required.
The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
From the given data for the enzyme dilution;
C1 = 1
C2 = 1/50 = 0.02
V1 = x
V2 = 5 ml
Making V1 subject of formula in the dilution formula:
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.
Learn more about dilution at: brainly.com/question/24709069
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A bimolecular reaction can be kinetically first order in behaviour provided one of the reactants is taken in such a large excess that its concentration may hardly change. Such a reactant will not contribute to the order. Thus, a bimolecular reaction will be of first order. These reactions are known as pseudo chemical reactions.
1. To solve this question, you need to equalize the mass of solute for both solution. The calculation would be:
mass of solute= volume*concentration
mass1=mass2
volume1 * concentration1 = volume2 * concentration2
volume1 * 0.7%= 2L *(1000ml/L) * 0.2%
volume1 = 2000ml * (0.2%/0.7%)
volume1= 571.429 ml
2. Since you already have the volume of stock needed, you just need to subtract it from the total solution volume to count the number of solvents needed.
new solution volume= stock volume + diluting solvent volume
2L * 2000ml/L = 571.429ml + diluting solvent volume
diluting solvent volume= 2000ml- 571.429 ml= 1428.571ml
PH is a simplified observation of the acidity of a solution, defined as the -log [H+] (negative logarithm of the hydrogen concentration).
-log[10^-4] = 4
The pH of this solution is 4.
Gravity is a force that can used draws everywhere, or everything to the Earth's center.
Hope it helped you.
-Charlie
Thanks!