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2 years ago

Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

Chemistry

1 answer:

Akimi4 [234]2 years ago

7 0

Answer:

Sulfosalicylic acid is used in urine tests to determine urine protein content.

Hope it's Helpful

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3 years ago

An element X exists in three forms A, B and C in the ratio 1:2:3. If C has 10 protonsand the number of neutrons in A, B and C ar

Strike441 [17]

x21 +ANSWER

(i) Ne-22

(ii)1s2s22p6

(iii)21.3

An element X exists in three forms A, B and C in the ratio 1:2:3. If C has 10 protons and the number of neutrons in A, B and C are 10, 11 and 12 respectively,Give the following:(i) Representation of form C of the element X(ii) Electronic configuration of form B of the element(iii) Calculate the average atomic mass.

(i)C has 10 protons and 12 neutrons so a mass of 10 +12 =22

element 10 is Neon (Ne) so this isotope is Ne-22

(ii) they all have the sane atomic number so the same number of electrons

with an electronic structure of 1s2s22p6

(iii) A weighs 20, B weighs 21, C weighs 22

the ratio is 1:2:3

weighted average weight is therefor

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3 years ago

At 298 K, the osmotic pressure of a glucose solution (C6H12O6 (aq)) is 12.1 atm. Calculate the freezing point of the solution. T

Anarel [89]

Answer: The freezing point of solution is -0.974°C

Explanation:

To calculate the concentration of solute, we use the equation for osmotic pressure, which is:

$\pi=iMRT$

where,

$\pi$ = osmotic pressure of the solution = 12.1 atm

i = Van't hoff factor = 1 (for non-electrolytes)

M = molarity of solute = ?

R = Gas constant = $0.0821\text{ L atm }mol^{-1}K^{-1}$

T = temperature of the solution = 298 K

Putting values in above equation, we get:

$12.1atm=1\times M\times 0.0821\text{ L.atm }mol^{-1}K^{-1}\times 298K\\\\M=\frac{12.1}{1\times 0.0821\times 298}=0.495M$

This means that 0.495 moles of glucose is present in 1 L or 1000 mL of solution

To calculate the mass of solution, we use the equation:

$\text{Density of substance}=\frac{\text{Mass of substance}}{\text{Volume of substance}}$

Density of solution = 1.034 g/mL

Volume of solution = 1000 mL

Putting values in above equation, we get:

$1.034g/mL=\frac{\text{Mass of solution}}{1000mL}\\\\\text{Mass of solution}=(1.034g/mL\times 1000mL)=1034g$

To calculate the number of moles, we use the equation:

$\text{Number of moles}=\frac{\text{Given mass}}{\text{Molar mass}}$

Moles of glucose = 0.495 moles

Molar mass of glucose = 180.16 g/mol

Putting values in above equation, we get:

$0.495mol=\frac{\text{Mass of glucose}}{180.16g/mol}\\\\\text{Mass of glucose}=(0.495mol\times 180.16g/mol)=89.18g$

Depression in freezing point is defined as the difference in the freezing point of pure solution and freezing point of solution.

The equation used to calculate depression in freezing point follows:

$\Delta T_f=\text{Freezing point of pure solution}-\text{Freezing point of solution}$

To calculate the depression in freezing point, we use the equation:

$\Delta T_f=iK_fm$

Or,

$\text{Freezing point of pure solution}-\text{Freezing point of solution}=i\times K_f\times \frac{m_{solute}\times 1000}{M_{solute}\times W_{solvent}\text{ (in grams)}}$

where,

Freezing point of pure solution = 0°C

i = Vant hoff factor = 1 (For non-electrolytes)

$K_f$ = molal freezing point elevation constant = 1.86°C/m

$m_{solute}$ = Given mass of solute (glucose) = 89.18 g

$M_{solute}$ = Molar mass of solute (glucose) = 180.16 g/mol

$W_{solvent}$ = Mass of solvent (water) = [1034 - 89.18] g = 944.82 g

Putting values in above equation, we get:

$0-\text{Freezing point of solution}=1\times 1.86^oC/m\times \frac{89.18\times 1000}{180.16g/mol\times 944.82}\\\\\text{Freezing point of solution}=-0.974^oC$

Hence, the freezing point of solution is -0.974°C

8 0

3 years ago

Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?​

Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?