Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
9) Substitution Reaction
10) Covalent Bond
11) Ionic Bond
12) Covalent Bond
13) Ionic Bond
14) 9 atoms
Explanation:
9) Substitution Reaction: Substitution reaction is a chemical reaction in which one atom, ion or species replaced by another atom, ion or species
10) Covalent Bond: Covalent bond is a bond that formed between two nonmetals, when both the species are non metal, the electronegativity of both the nonmetals are comparatively same, hence any of both do not pulls completely electron of other & the bond is formed by the sharing of electron.
11) Ionic Bond: We know that nonmetals have high electronegativity than those of metals, due to high electronegativity non metals pulls the electrons of metals but there is enough interaction that non metal do not escape after pulling the electron, & an ionic bond generates where non metals possess negative charge & positive charge goes to metal.
12) Covalent Bond: The bond formed between two atoms having less electronegativity diffrence by sharing of electron pair is know as covalent bond. for e.g the Carbon - Hydrogen bond in methane (CH4) molecule is covalent bonded because the electronegativity of carbon is 2.5 & that of hydrogen is 2.1 which is almost close, hence the bond formed is covalent.
13) Ionic Bond: The bond formed between two atoms having high electronegativity diffrence & the bond formed is due to complete transfer of electron by one species. For e.g. NaCl the sodium is a metal having electronegativity 0.9 and chlorine is non metal having electronegativity 3.0 the electronegativity diffrence is too high, hence the chlorine behaves as Cl- ion that of sodium as Na+, both the components behaves as ion but they are bonded &that bond is called as Ionic bond.
14) 9 Atoms: One molecule of water (H2O) posses three atoms, two hydrogen atoms & one oxygen atom, the number of atoms in 3 molecules of water 3×3 = 9 atoms.
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Answer:
c
Explanation:
i read a artical on him and it said
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Answer:
D.HI
Explanation:
because this is the most different
Answer:
The correct answer is (b)
Explanation:
Charles law describes the behavior of gases when heated. Charles law states that the volume of a given mass of gas would increase as its Kelvin temperature increases provided the pressure is held constant. That is the volume of a given mass of gas is directly proportional to its Kelvin temperature at constant pressure
