Question

Open Ended Identify factors that can influence enzyme activity. Describe the effect of each.

Answer 1

Temperature, pH, enzyme concentration, substrate concentration, and the existence of any inhibitors or activators are a few of the variables that influence enzyme activity.

• The rate of the reaction increases proportionally as the enzyme concentration rises. When diagnosing disorders, this feature is utilized to gauge the serum enzymes' activity.

• The enzymes are highly sensitive to heat changes because they are proteins. Unlike typical chemical processes, enzyme activity is temperature-dependent. Each enzyme has a certain temperature range where it is more active, as you have seen. The ideal temperature, which falls between 37 and 40 degrees Celsius, is at this point.

• When there is a certain amount of enzyme present, the rate of the enzymatic reaction rises as the concentration of the substrate rises until a limiting rate is achieved, after which additional increases in the concentration of the substrate have no effect on the reaction rate.

• The most accurate way to estimate a solution's hydrogen ion concentration (H+) is to use the potential of hydrogen (pH). Additionally, it establishes whether the liquid is basic, acidic, or neutral. In general, liquids with a pH of 7 or lower are classified as acids, whereas liquids with a pH of 7 or higher are classified as bases or alkalines.

• For optimal activity, several enzymes need specific inorganic metallic cations, such as Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, and K+. Anions are also occasionally required for enzymes.

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