<u>Temperature, pH, enzyme concentration, substrate concentration, and the existence of any inhibitors or activators</u> are a few of the variables that influence enzyme activity.
The rate of the reaction increases proportionally as the enzyme concentration rises. When diagnosing disorders, this feature is utilized to gauge the serum enzymes' activity.
The enzymes are highly sensitive to heat changes because they are proteins. Unlike typical chemical processes, enzyme activity is temperature-dependent. Each enzyme has a certain temperature range where it is more active, as you have seen. The ideal temperature, which falls between 37 and 40 degrees Celsius, is at this point.
When there is a certain amount of enzyme present, the rate of the enzymatic reaction rises as the concentration of the substrate rises until a limiting rate is achieved, after which additional increases in the concentration of the substrate have no effect on the reaction rate.
The most accurate way to estimate a solution's hydrogen ion concentration (H+) is to use the potential of hydrogen (pH). Additionally, it establishes whether the liquid is basic, acidic, or neutral. In general, liquids with a pH of 7 or lower are classified as acids, whereas liquids with a pH of 7 or higher are classified as bases or alkalines.
For optimal activity, several enzymes need specific inorganic metallic cations, such as Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, and K+. Anions are also occasionally required for enzymes.
