200ml of 0.1M tris-H with 732ml water and 68ml of 0.1M NaOH
<h3>What is the purpose of tris hydroxymethyl aminomethane?</h3>
The Food and Drug Administration has approved THAM, a rarely used buffer, for the treatment of metabolic acidosis related to cardiac bypass surgery and metabolic acidosis related to cardiac arrest.
<h3>What use does Tris buffer serve?</h3>
- For DNA agarose electrophoresis, Tris buffers are frequently employed. TBE (Tris borate/EDTA) and TAE (Tris acetate/EDTA) are the two major buffers.
- These Tris buffers can normally be used interchangeably, despite some variations in the resolution of various types of DNA and their mobility during electrophoresis.
- Using a tris buffer helps keep the pH steady during cell lysis and extraction because DNA extraction is a pH-sensitive procedure.
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the question you are looking for is
Tris-hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical research. Shown here is the structure of TRIS in its protonated form: Its acid dissociation constant, pKa, is 8.32 x10-9 M. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 Msolutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1 L solution of 0.02 M TRIS buffer, pH 7.8.
