All categories

2 years ago

Why would you need a locating agent in an experiment to separate amino acids by chromatography

1 answer:

Nadusha1986 [10]2 years ago

5 0

A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.

<h3>What is chromatography?</h3>

Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.

There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.

<h3>What is the use of a locating agent in Chromatography?</h3>

In paper chromatography, colorless compounds are examined using a locating agent.

It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.

Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.

Learn more about chromatography:

brainly.com/question/11960023

#SPJ9

You might be interested in

Which option describes the direction of thermal energy transfer in earth's oceans?

BlackZzzverrR [31]

Answer:

Energy is moved from areas of surplus to those of deficit, with warm currents transporting warm water polewards and cold currents taking colder water to lower latitudes. It holds onto this heat for longer than the land does and the ocean currents move this heat around, from the tropics to higher latitudes.

3 0

3 years ago

Read 2 more answers

The hydrogen ion concentration of a vinegar solution is 0.00010 m. how is this concentration written in scientific notation?

9966 [12]

We are given with a vinegar with a hydrogen ion concentration of 0.00010 m. We are asked to express this concentration in scientific notation. The answer when expressed in scientific notation is 1x10^-4 m or molality. Answer is <span>1x10^-4 m. </span>

3 0

4 years ago

Read 2 more answers

Why aren’t dominant genes ALWAYS more common?

Zolol [24]

Answer:

selective breeding

Explanation:

Example: pretend that rabbits can only be brown or black and black is dominant. If only the brown rabbits reproduce it will eventually be more common Hope this helps

5 0

3 years ago

Which statement about Schrödinger's theory of the atomic model is true?

vekshin1

Answer : Option C) It explained the odds of finding the position of an electron.

Explanation : Schrödinger's theory of the atomic model is true for the probability of finding the electron around the nucleus. Schrödinger used odd mathematical equations to describe the likelihood of finding an electron in a certain position around the nucleus. This atomic model was known as the quantum mechanical model of the atom. He posed an equation which is now called as the Schordinger’s Wave Equation, which incorporates both the wave-like and particle-like behaviors of electrons, which stated that electrons can act like waves/light and particles. Which later helped to develop the uncertainty principle for finding the probable position of the electron.

4 0

4 years ago

If 30.0 mL of Ca(OH)2 with an unknown concentration is neutralized by 40.0 mL of 0.175 M HCl, what is the concentration of the C

Over [174]

Answer:

Concentration of Ca(OH)₂:

0.117 M.

Explanation:

How many moles of HCl is consumed?

Note the unit of concentration: moles per liter solution.

$c(\text{HCl}) = 0.175\;\text{M} = 0.175\;\text{mol}\cdot\textbf{L}^{-1}$ .

Convert milliliters to liters.

$V(\text{HCl})=40.0\;\text{mL} = 0.0400\;\text{L}$ .

$n(\text{HCl}) = c(\text{HCl})\cdot V(\text{HCl})= 0.175\;\text{mol}\cdot\text{L}^{-1} \times 0.0400\;\text{L}= 7.00\times 10^{-3}\;\text{mol}$ .

How many moles of NaOH in the solution?

Refer to the equation. The coefficient in front of Ca(OH)₂ is 1. The coefficient in front of HCl is 2. In other words, it takes two moles of HCl to neutralize one mole of Ca(OH)₂. That $7.00\times 10^{-3}\;\text{mol}$ of HCl will neutralize only half that much Ca(OH)₂.

$\displaystyle n(\text{Ca}(\text{OH})_2)=\frac{1}{2}\;n(\text{HCl}) = 3.50\times 10^{-3}\;\text{mol}$ .

What's the concentration of the Ca(OH)₂ solution?

Concentration is the number of moles of solute per unit volume.

$\displaystyle c(\text{Ca}(\text{OH})_2) = \frac{n(\text{Ca}(\text{OH})_2)}{V(\text{Ca}(\text{OH})_2)} = \frac{3.50\times 10^{-3}\;\text{mol}}{0.0300\;\text{L}}=0.117\;\text{mol}\cdot\text{L}^{-1}$ .

3 0

3 years ago