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pychu [463]
2 years ago
11

Why would you need a locating agent in an experiment to separate amino acids by chromatography

Chemistry
1 answer:
Nadusha1986 [10]2 years ago
5 0

A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.

<h3>What is chromatography?</h3>

Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.

There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.

<h3>What is the use of a locating agent in Chromatography?</h3>

In paper chromatography, colorless compounds are examined using a locating agent.

It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.

Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.

Learn more about chromatography:

brainly.com/question/11960023

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Explanation:

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The mole is defined as the amount of a substance containing the same number of particles as exactly 12 g of C-12. The amu is def
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Because it wouldn't make any sense

Explanation:

First of all, I think it's important to highlight the definition of isotope.

Isotope (Wikipedia): Variants of a particular chemical element <u>which differ in neutron number</u>...

This means that two isotopes are the same element but have different net mass per atom, due to the different number of neutrons.

Therefore, it's important to make the definition on the same isotope so that the proportion is equal. If the definition would be made on different isotopes, the proportion wouldn't have any sense. Let me be clear with this example:

mass in grams of a C-12 atom = 1.9944235 × 10 ^ -23 g  --> this is the mass of a single C atom.

By definition --> 1 mol of anything = 6.02 x 10 ^ 23 particles of anything

Therefore, we know how much a single C atom weights. How many grams do you think that 6.02 x 10 ^ 23 atoms of C (i.e a mol of C) could weight??

1 single C atom ----------------------------- 1.9944235 × 10 ^ -23 g

6.02 x 10 ^ 23 atoms of C   ------------ <u>12.006 g  !!</u>

These 12 g is the same quantity than above! Therefore, 1 mol of C weights 12 g. If the definition were made with 13 g of C-13 (the other C isotope), these numbers will not be the same --> There would be a contradiction.

Regarding the second question, we need to search Ne-20 atomic mass in grams -->  3,3509177 × 10 ^ -23 g

Hence, if we follow the same rule, the amu would be 1/12 of Ne-20.

[ 3,3509177 × 10 ^ -23 g ] / 12 = 2.79 ^ -24 g

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3 years ago
Which statements correctly describe atmospheric pressure?
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3. It can be measured in atmospheres or kilopascals.

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Atmospheric pressure decreases with increasing height, because there are fewer air molecules above a given object.

Barometer is an instrument used in meteorology to measure atmospheric pressure.

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