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pychu [463]
1 year ago
11

Why would you need a locating agent in an experiment to separate amino acids by chromatography

Chemistry
1 answer:
Nadusha1986 [10]1 year ago
5 0

A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.

<h3>What is chromatography?</h3>

Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.

There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.

<h3>What is the use of a locating agent in Chromatography?</h3>

In paper chromatography, colorless compounds are examined using a locating agent.

It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.

Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.

Learn more about chromatography:

brainly.com/question/11960023

#SPJ9

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An atom of iron has the atomic number 26 and a mass number of 56. how many protons plus neutrons are in the nucleus?
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Give the values of n, l and ml for: (a) each orbital in the 2p subshell, (b) each orbital in the 5d subshell.?
SCORPION-xisa [38]

Hey there!:

* For 2p subshell :

n = 2, l =1, ml = -1, 0, +1

* for 5d subshell,

n = 5, l = 2, ml = -2, -1, 0, +1, +2


Hope that helps!

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What volume of 0.200 M H2SO* will be needed to neutralize<br><br> 50.00 mL of 0.178 MNaOH?
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Answer:

10.00 mL

Explanation:

Please, do not forget to write the dot and 2 of Zeros after the number 10 in the answer!

Please see the step-by-step solution in the picture attached below.

Hope this answer can help you. Have a nice day!

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Which determines how atoms combine to form molecules?
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In E. coli, the enzyme hexokinase catalyzes the reaction: Glucose + ATP → glucose 6-phosphate + ADP The equilibrium constant, Ke
kondor19780726 [428]

Answer:

Explanation:

Glucose + ATP → glucose 6-phosphate + ADP The equilibrium constant, Keq, is 7.8 x 102.

In the living E. coli cells,

[ATP] = 7.9 mM;

[ADP] = 1.04 mM,

[glucose] = 2 mM,

[glucose 6-phosphate] = 1 mM.

Determine if the reaction is at equilibrium. If the reaction is not at equilibrium, determine which side the reaction favors in living E. coli cells.

The reaction is given as

Glucose + ATP → glucose 6-phosphate + ADP

Now reaction quotient for given equation above is

q=\frac{[\text {glucose 6-phosphate}][ADP]}{[Glucose][ATP]}

q=\frac{(1mm)\times (1.04 mm)}{(7.9mm)\times (2mm)} \\\\=6.582\times 10^{-2}

so,

q ⇒ following this criteria the reaction will go towards the right direction ( that is forward reaction is favorable  until q = Keq

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