Why would you need a locating agent in an experiment to separate amino acids by chromatography

1 answer:

Nadusha1986 [10]2 years ago

5 0

A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.

<h3>What is chromatography?</h3>

Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.

There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.

<h3>What is the use of a locating agent in Chromatography?</h3>

In paper chromatography, colorless compounds are examined using a locating agent.

It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.

Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.

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liquid octane(CH3)(CH2)6CH3) reacts with gaseous oxygen gas(O2) to produce gaseous carbon dioxide(CO2) and gaseous water(H2O). I

max2010maxim [7]

The percent yield of carbon dioxide will be 49.0 %.

<h3>Percent yield</h3>

First, let's look at the equation of the reaction:

$2C_8H_1_8 + 25O_2 -- > 16CO_2 + 18H_2O$

The mole ratio of octane to oxygen is 2:25.

Mole of 3.43 g octane = 3.43/114.23 = 0.03 mol

Mole of 19.1 g oxygen = 19.1/32 = 0.60 mol

Thus, octane is limiting.

Mole ratio of octane to carbon dioxide = 2:16.

Equivalent mole of carbon dioxide = 0.03 x 8 = 0.24 mol

Mass of 0.24 mol carbon dioxide = 0.24 x 44.01 = 10.5624 grams

Percent yield of carbon dioxide = 5.18/10.5624 = 49.0 %

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2 years ago

What is the color of the indicator thymol blue in a solution that has a ph of 11?.

Leviafan [203]

Answer:

Red

Explanation:

Red color is evidenced when thymol blue indicator is in a solution having a pH of 11. A pH of 11 means that the solution is basic or alkaline. Therefore, the indicator turns red, indicating that the solution is alkaline.

When thymol blue indicator is in a acidic solution, the indicator remains blue.

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2 years ago

The form of heat transfer taking place in the crust of the earth would be ____

Anna [14]

The form of heat transfer taking place in the crust of the Earth would be Convection.

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3 years ago

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11111nata11111 [884]

The answer is A.......