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1 year ago

Why would you need a locating agent in an experiment to separate amino acids by chromatography

1 answer:

Nadusha1986 [10]1 year ago

5 0

A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.

<h3>What is chromatography?</h3>

Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.

There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.

<h3>What is the use of a locating agent in Chromatography?</h3>

In paper chromatography, colorless compounds are examined using a locating agent.

It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.

Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.

Learn more about chromatography:

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The powder mixture (Cu, Al and Fe) = 10g was oxidized from sufficient chloride acid. 1) What are the possible reactions to the p

Mandarinka [93]

Answer:

1) 2Al + 6HCl ⟶ 2AlCl₃ + 3H₂

Fe + 2HCl ⟶ FeCl₂ + H₂

2) Cu = 2.5 g; Al = 3.5 g; Fe = 4.0 g

Explanation:

1) Possible reactions

2Al + 6HCl ⟶ 2AlCl₃ + 3H₂

Fe + 2HCl ⟶ FeCl₂ + H₂

2) Mass of each metal

a) Mass of Cu

The waste was the unreacted copper.

Mass of Cu = 2.5 g

b) Masses of Al and Fe

We have two relations :

Mass of Al + mass of Fe = 10 g - 2.5 g = 7.5 g

H₂ from Al + H₂ from Fe = 6.38 L at NTP

i) Calculate the moles of H₂

NTP is 20 °C and 1 atm.

$\begin{array}{rcl}pV & = & n RT\\\text{1 atm} \times \text{6.38 L} & = & n \times 0.08206 \text{ L}\cdot\text{atm}\cdot\text{K}^{-1}\text{mol}^{-1} \times \text{293.15 K}\\6.38 & = & 24.06n \text{ mol}^{-1} \\n & = & \dfrac{6.38}{24.06 \text{ mol}^{-1} }\\\\ & = & \text{0.2652 mol}\\\end{array}$

(ii) Solve the relationship

Let x = mass of Al. Then

7.5 - x = mass of Fe

Moles of Al = x/27

Moles of Fe = (7.5 - x)/56

Moles of H₂ from Al = (3/2) × Moles of Al = (3/2) × (x/27) = x /18

Moles of H₂ from Fe = (1/1) × Moles of Fe = (7.5 - x)/56

∴ x/18 + (7.5 - x)/56 = 0.2652

56x + 18(7.5 - x) = 267.3

56x + 135 - 18x = 267.3

38x = 132.3

x = 3.5 g

Mass of Al = 3.5 g

Mass of Fe = 7.5 g - 3.5 g = 4.0 g

The masses of the metals are Cu = 2.5 g; Al = 3.5 g; Fe = 4.0 g

4 0

3 years ago

Which of the following could be considered a scientific method? 01.01 the science of chemistry

d1i1m1o1n [39]

Answer:

a) the ability to extend the theory to make predictions

b) it is explanatory

Explanation:

Could be a Hallmark of a Scientific Theory: a & b

-Quizlet

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2 years ago

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What method would you use to obtain pure water from a mixture of water and ink

mel-nik [20]

Answer: Distillation

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PF3Br2 is a nonpolar molecule. Based on this information, determine the F−P−F bond angle, the Br−P−Br bond angle, and the F−P−Br

inna [77]

Answer:

120,180,90.

Explanation:

PF3Br2 has a trigonal bypiramidal geometry. The three atoms of F will be arranged around the P atom in the same plane and each F-P-F bond will have an angle of 120 degrees. There will be only one Br atom above and beneath the P atom so the bond angle for Br-P-Br will be 180 degrees. Finally, the F−P−Br bond angle will be at 90 degrees.

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Tresset [83]

Lake Victoria

Explanation:

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