Explanation:
The given reaction is as follows.
![E + S \rightleftharpoons ES \xrightarrow[]{k_{2}} E + P](https://tex.z-dn.net/?f=E%20%2B%20S%20%5Crightleftharpoons%20ES%20%5Cxrightarrow%5B%5D%7Bk_%7B2%7D%7D%20E%20%2B%20P)
Here, [E] = triose phosphate isomerase = 0.1 
[S] = Dihydroxy acetone phosphate = 5 
[P] = Glyceraldehyde-3-phosphate = 2 
Therefore, velocity of the reaction will be as follows.
v =
= ![\frac{K_{2}[E][S]}{K_{M} + [S]}](https://tex.z-dn.net/?f=%5Cfrac%7BK_%7B2%7D%5BE%5D%5BS%5D%7D%7BK_%7BM%7D%20%2B%20%5BS%5D%7D)
where,
= Michaelic menten constant = 
v = 
= 
or, = 30 nm/s
Hence, we can conclude that the actual velocity of the forward reaction under physiologic conditions if KM = 10 μM is 30 nm/s.
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