Question

What is the unit of measurement used to describe the size of a protein in gel electrophoresis?.

Answer 1

Daltons, a unit of molecular weight, are used to measure protein size. The mass of a hydrogen atom, which is 1.66 x 10-24 grammes, is considered to be one Dalton. Since the majority of proteins have masses in the thousands of Dalton range, the term kilodalton (kDa) is frequently used to refer to the molecular weight of proteins.

• Daltons (Da) or kilodaltons, which are units of molecular weight, are typically used to measure proteins (kDa). By comparing the sizes of standard molecular weight markers of known size that were run in parallel with the unknown samples during gel electrophoresis, one can estimate the sizes of DNA, RNA, or protein molecules that were separated on gels.
• Different techniques have been used to assess the electrophoretic mobilities of DNA polymer fragments in an agarose gel and then convert those measurements to lengths using the reciprocal method. The length estimations can be greatly distorted by the measurement technique.

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