Hey there!:
From the given data ;
Reaction volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )
Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:
[E]t in molar = g/L * mol/g
[E]t = 0.01 g/L * 1 / 45,000
[E]t = 2.22*10⁻⁷
Vmax = 0.758 umole/min/ per mL
= 758 mmole/L/min
=758000 mole/L/min => 758000 M
Therefore :
Kcat = Vmax/ [E]t
Kcat = 758000 / 2.2*10⁻⁷ M
Kcat = 3.41441 *10¹² / min
Kcat = 3.41441*10¹² / 60 per sec
Kcat = 5.7*10¹⁰ s⁻¹
Hence kcat of xyzase is 5.7*10¹⁰ s⁻¹
Hope that helps!
17 protons 17 electrons 18 neutrons
Answer:
0.2 mL stock solution, 0.8 solvent, 0.1 mL first solution and 0.9 solvent
Explanation:
The final volume for fist solution is 1 mL and concentration must will be 1/5, then 1 mL/5=0.2 mL. For complete the 1 mL add the missing solvent volume 1 mL-0.2 mL=0.8 mL. For second solution, assuming final volume is 1 mL, and concentration 1/10, then we have 1 mL /10=0.1 mL solution 1/5. Completing volume, 1 mL-0.1 mL= 0.9 mL solvent.
Answer:
where is the option I can't see plz give the option first the I try to give answer
