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Volgvan
4 years ago
6

Which of the following statements regarding Anfinsen's denaturing experiments with ribonuclease A (RNAse A) are valid? Exposing

the denatured protein to air oxidation and then dialysis to remove urea restored the protein to its original functionality. Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality. Denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein. Anfinsen concluded that protein folding is determined by its primary sequence.
Chemistry
1 answer:
netineya [11]4 years ago
7 0

Answer:

B, C, and D

Explanation:

Anfinsen found that denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein.  

Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality.

He concluded that protein folding is determined by its primary sequence.

A is false. Anfinsen removed the urea first and then allowed the enzyme solution to re-oxidize slowly in air.

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The prefab question someone help please !
lina2011 [118]

Answer:   " 0.69 g / mL "

____________________________________________

Explanation:  

______________________________________________

The "pre-lab question" given is:

_____________________________________________

"The volume of an unknown liquid is 15 ml, and the mass of the liquid and the graduated cylinder it is in is 55.2g.   If the mass of the graduated cylinder is 44.8g , what is the density of the unknown liquid? " .

____________________________________________

Note:  The volume of the unknown liquid is 15 mL ;  regardless of whether or not the "unknown liquid" is in the graduated cylinder.

The density of the unknown liquid is measured in:  "g / mL " ;  

  that is, "grams per mL" .

____________________________________________

Note:  "D = m / V " ;   that is;  "Density  = mass/ volume" ;

                                  that is:   ["Density = mass per 'unit volume' ]" .

____________________________________________

So;  to find the density, "D" , of the "unknown liquid" ;  we would have to find the "mass" of the "unknown liquid" by "subtracting" :

       the "known mass of liquid when the liquid is not in the cylinder";  that is:  " 44.8g" ;  From:

       the "known combined value of the: 'mass of the liquid PLUS the mass of the cylinder" ;  that is:  "55.2g" ;

→     " 55.2g - 44.8g = 10.4g " .

___________________________________________

So:   " D =  m / V " ; (55.2g - 44.8 g) / 15 mL  " ;

              =  (55.2g - 44.8 g) / 15 mL  " ;

              =  (10.4g) / 15mL  ;

____________________________________________

Note that the "Density" =  mass per "unit volume" ;

→ So:  D = m / V ;  in units of:  " g / mL " (grams per millileter) ;

            = (10.4g) / 15mL  ;

             = [ (10.4) / 15 }  g/ mL ;  

            =  0.693333333333333.... g / mL  ;

       →  We round to "2 (two) significant figures" ;

       → since we have:  "10.4 g / 15 mL " ;

             and 15 mL is considered a measured/experimental value;

             and:  10.4g is considered a measured/experimental value;

→  so, the least precise value;  15 mL (has only 2 (two) significant figures ;

      compared to other value:  10.4g (which has 3 (three) significant figures;

→  so we shall round off to 2 (two) significant figures:

             =  0.69 g / mL

____________________________________________

              →  which is our answer:  " 0.69 g / mL " .

____________________________________________

3 0
3 years ago
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