Before the development of electrophoresis to separate macromolecules, high-speed centrifugation was used to isolate DNA.
A laboratory procedure called electrophoresis is used to divide DNA, RNA, or protein molecules according to their size and electrical charge. The molecules are moved by an electric current through a gel or other matrix. The technology of electrophoresis is crucial for the separation and examination of nucleic acids. At the lab bench, cloned DNA fragments are frequently isolated and worked with using nucleic acid electrophoresis.
High-speed centrifugation employs centrifugal force to separate particles with various densities or masses suspended in a liquid. High-speed rotation of the solution inside the tube causes each particle's angular momentum to experience centrifugal forces inversely proportionate to its mass.
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Answer:
(a) 7.11 x 10⁻³⁷ m
(b) 1.11 x 10⁻³⁵ m
Explanation:
(a) The de Broglie wavelength is given by the expression:
λ = h/p = h/mv
where h is plancks constant, p is momentum which is equal to mass times velocity.
We have all the data required to calculate the wavelength, but first we will have to convert the velocity to m/s, and the mass to kilograms to work in metric system.
v = 19.8 mi/h x ( 1609.34 m/s ) x ( 1 h / 3600 s ) = 8.85 m/s
m = 232 lb x ( 0.454 kg/ lb ) = 105.33 kg
λ = h/ mv = 6.626 x 10⁻³⁴ J·s / ( 105.33 kg x 8.85 m/s ) = 7.11 x 10⁻³⁷ m
(b) For this part we have to use the uncertainty principle associated with wave-matter:
ΔpΔx > = h/4π
mΔvΔx > = h/4π
Δx = h/ (4π m Δv )
Again to utilize this equation we will have to convert the uncertainty in velocity to m/s for unit consistency.
Δv = 0.1 mi/h x ( 1609.34 m/mi ) x ( 1 h/ 3600 s )
= 0.045 m/s
Δx = h/ (4π m Δv ) = 6.626 x 10⁻³⁴ J·s / (4π x 105.33 kg x 0.045 m/s )
= 1.11 x 10⁻³⁵ m
This calculation shows us why we should not be talking of wavelengths associatiated with everyday macroscopic objects for we are obtaining an uncertainty of 1.11 x 10⁻³⁵ m for the position of the fullback.
Answer:
We need 0.375 mol of CH3OH to prepare the solution
Explanation:
For the problem they give us the following data:
Solution concentration 0,75 M
Mass of Solvent is 0,5Kg
knowing that the density of water is 1g / mL, we find the volume of water:

Now, find moles of
are needed using the molarity equation:
therefore the solution is prepared using 0.5 L of H2O and 0.375 moles of CH3OH, resulting in a concentration of 0,75M
Answer:
Aluminium is ordinarily classified as a metal. It is lustrous, malleable and ductile, and has high electrical and thermal conductivity. Like most metals, it has a close-packed crystalline structure and forms a cation in an aqueous solution.