It’s a polyatomic and it has a negative charge. It’s located in the nucleus of the atom, along with the protons.
One of the best buffer choice for pH = 8.0 is Tris with Ka value of 6.3 x 10^-9.
To support this answer, we first calculate for the pKa value as the negative logarithm of the Ka value:
pKa = -log Ka
For Tris, which is an abbreviation for 2-Amino-2-hydroxymethyl-propane-1,3 -diol and has a Ka value of 6.3 x 10^-9, the pKa is
pKa = -log Ka
= -log (6.3x10^-9)
= 8.2
We know that buffers work best when pH is equal to pKa:
pKa = 8.2 = pH
Therefore Tris would be a best buffer at pH = 8.0.
Answer:
substitution is the best method or collecting like terms
Explanation: