Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
The atomic radius corresponds to Sigma
Explanation:
It is the Van Der Waals radius ;)
Answer:
Cement is a combination of calcium, silicone, aluminum, iron and lots of other ingredients to make side walks, home made crafts, etc. The person who invented concrete is Joseph Aspdin.
Explanation:
Answer:
They went from atoms to oxygen molecule.
Explanation:
I'm not sure if this is the answer your teacher is looking for, but in simple terms you had 2 oxygen atoms. Together they make an oxygen molecule, which is the stuff we breathe.
Answer:
5.17.
Explanation:
<em>∵ [H₃O⁺][OH⁻] = 10⁻¹⁴.
</em>
[OH⁻] = 1.5 x 10⁻⁹ M.
∴ [H₃O⁺] = 10⁻¹⁴/[OH⁻] = 10⁻¹⁴/(1.5 x 10⁻⁹ M) = 6.66 × 10⁻⁶ M.
∵ pH = - log[H₃O⁺]
<em>∴ pH = - log(6.66 × 10⁻⁶ M) = 5.17.
</em>
