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tekilochka [14]
3 years ago
8

Tyrosine absorbs maximally at 290 nm. A 0.2162 g sample containing some amount of Tyrosine was placed into a 250.0 mL volumetric

flask and diluted to the mark. This solution had an absorbance of 0.118 (at 290 nm) in a 2.00 cm pathlength cell. A sample of pure Tyrosine (MW - 1818 g/mole) weighing 12.4 mg was dissolved in a 100.0 mL of solution. A 10.0 mL aliquot of this 100.0 mL solution was added to a 250.0 mL flask and diluted to the mark. This last diluted sample had an absorbance of 0.473 at 290 nm in a 1.00 cm cell. Calculate the weight percent of Tyrosine in the sample. Assume no other compounds in the sample, except Tyrosine, absorb at 290 nm.A. 0.14 %
B. 0.069 %
C. 0.028 %
D. 0.040 %
E. 1.80 %
F. 2.63 %

Chemistry
1 answer:
mojhsa [17]3 years ago
7 0

Answer:

B. 0.069 %

Explanation:

It should be initially noted in this answer in particular that, the amino acids tryptophan and tyrosine have a very precise 280 nm absorption rate, which allows a direct A280 size of protein concentration. The 280 nm UV absorbance rate is regularly utilized to approximate protein concentration in laboratories due to its simplistic nature, its affordability and also the ease of usage.

kindly check the attached image below for the solution to the above question.

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Explanation:

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Suppose 110.0 mL110.0 mL of hydrogen gas at STP combines with a stoichiometric amount of fluorine gas and the resulting hydrogen
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Suppose 110.0 mL of hydrogen gas at STP combines with a stoichiometric amount of fluorine gas and the resulting hydrogen fluoride dissolves in water to form 150.0 mL of an aqueous solution. 0.032 M is the concentration of the resulting hydrofluoric acid.

<h3>What is Balanced Chemical Equation ?</h3>

The balanced chemical equation is the equation in which the number of atoms on the reactant side is equal to the number of atoms on the product side in an equation.

Now write the balanced chemical equation

H₂ + F₂ → 2HF

<h3>What is Ideal Gas ?</h3>

An ideal gas is a gas that obey gas laws at all temperature and pressure conditions. It have velocity and mass but do not have volume. Ideal gas is also called perfect gas. Ideal gas is a hypothetical gas.

It is expressed as:

PV = nRT

where,

P = Pressure

V = Volume

n = number of moles

R = Ideal gas constant

T = temperature

Here,

P = 1 atm   [At STP]

V = 110 ml = 0.11 L

T = 273 K   [At STP]

R = 0.0821   [Ideal gas constant]

Now put the values in above expression

PV = nRT

1 atm × 0.11 L = n × 0.0821 L.atm/ K. mol × 273 K

n = \frac{1\ \text{atm} \times 0.11\ L}{0.0821\ \text{L. atm/ K. mol} \times 273\ K}

n = 0.0049 mol

<h3>How to find the concentration of resulting solution ? </h3>

To calculate the concentration of resulting solution use the expression

C = \frac{n}{V}

   = \frac{0.0049}{0.15}  

   = 0.032 M

Thus from the above conclusion we can say that Suppose 110.0 mL of hydrogen gas at STP combines with a stoichiometric amount of fluorine gas and the resulting hydrogen fluoride dissolves in water to form 150.0 mL of an aqueous solution. 0.032 M is the concentration of the resulting hydrofluoric acid.

Learn more about the Ideal Gas here: brainly.com/question/25290815
#SPJ4

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2 years ago
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