Answer: <u>Endonuclease enzymes used in molecular biology that cut DNA at specified points.</u>
Explanation:
Enzymes are specific protein types which bind to a substrate within a reaction, to increase the rate of reaction within the solution- they speed up the rate of reaction.
Restriction enzymes are bacteria-derived enzymes; these make cuts on deoxyribonucleic acid molecules or DNA. These are also called restriction endonucleases. They are utilized in molecular biology for DNA cloning and sequencing and cut DNA into smaller pieces called fragments.
Restriction enzymes make directed cuts on DNA molecules. They precisely target sites on DNA to produce mostly identical or homogenous, discrete fragments of equal sizes, producing blunt or sticky ends. In order to do this, they recognize sequences of nucleotides that correspond with a complementary sequence on the endonuclease called restriction sites.
There are several kinds that may require cofactors (chemical or metallic compounds that aid in enzyme activity) :
- Type I: cleave far away from the recognition site; require ATP and SAMe S-Adenosyl-L-Methionine
- Type II: cleave near to the site; require Magnesium
- Type III: cleave near to the site; require ATP which is not hydrolysed but SAMe S-Adenosyl-L-Methionine is optional
- Type IV: cleavage targeted to DNA that have undergone post transcriptional modification through certain types of methylation (addition of a methyl group)
Zinc would be considered the strongest reducing agent.
<h3>Reducing agent</h3>
A reducing agent is a chemical species that "donates" one electron to another chemical species in chemistry (called the oxidizing agent, oxidant, oxidizer, or electron acceptor). Earth metals, formic acid, oxalic acid, and sulfite compounds are a few examples of common reducing agents.
Reducers have excess electrons (i.e., they are already reduced) in their pre-reaction states, whereas oxidizers do not. Usually, a reducing agent is in one of the lowest oxidation states it can be in. The oxidation state of the oxidizer drops while the oxidizer's oxidation state, which measures the amount of electron loss, increases. The agent in a redox process whose oxidation state rises, which "loses/donates electrons," which "oxidizes," and which "reduces" is known as the reducer or reducing agent.
Learn more about reducing agent here:
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Answer:
Therefore it will take 7.66 hours for 80% of the lead decay.
Explanation:
The differential equation for decay is


Integrating both sides
ln A= kt+c₁

[
]
The initial condition is A(0)= A₀,


.........(1)
Given that the
has half life of 3.3 hours.
For half life
putting this in equation (1)

[taking ln both sides,
]

⇒k= - 0.21
Now A₀= 1 gram, 80%=0.8
and A= (1-0.8)A₀ = (0.2×1) gram = 0.2 gram
Now putting the value of k,A and A₀in the equation (1)




⇒ t≈7.66
Therefore it will take 7.66 hours for 80% of the lead decay.
Answer:
Bond energy of carbon-fluorine bond is 485 kJ/mol
Explanation:
Enthalpy change for a reaction, is given as:
![\Delta H_{rxn}=\sum [n_{i}\times (E_{bond})_{i}]-\sum [n_{j}\times (E_{bond})_{j}]](https://tex.z-dn.net/?f=%5CDelta%20H_%7Brxn%7D%3D%5Csum%20%5Bn_%7Bi%7D%5Ctimes%20%28E_%7Bbond%7D%29_%7Bi%7D%5D-%5Csum%20%5Bn_%7Bj%7D%5Ctimes%20%28E_%7Bbond%7D%29_%7Bj%7D%5D)
Where
and
represents average bond energy in breaking "i" th bond and forming "j" th bond respectively.
and
are number of moles of bond break and form respectively.
In this reaction, one mol of C=C, four moles of C-H and one mol of F-F bonds are broken. One mol of C-C bond, four moles of C-H bonds and two moles of C-F bonds are formed
So, 
or, 
or, 
So bond energy of carbon-fluorine bond is 485 kJ/mol
Answer:
44.2 L
Explanation:
Use Charles Law:

We have all the values except for V₂; this is what we're solving for. Input the values:
- make sure that your temperature is in Kelvin
From here, we need to get V₂ by itself. To do this, multiply by 273 on both sides:

Therefore, V₂ = 44.2 L
It's also helpful to know that temperature and volume are linearly related. So, when temperature drops, so will volume and vice versa.