In a flame photometric analysis, salt solution is first vaporized using the heat of flame, followed by this electrons from valance shell gets excited from ground state to excited state. Followed by this de-excitation of electron bring backs electrons to ground state. This process is accompanied by emission of photon. The photon emitted is characteristic of an element, and number of photons emitted can be used for quantitative analysis.
<span>Following are the investigative question that you can answer by doing this experiment.
</span>1) What information can be obtained from the colour of flame?
2) <span>State the relationship between wavelength, frequency, and energy?
</span><span>3) Can you identify the metal present in unknown sample provided?
4) How will you identify amount of metal present in sample solution?
5) </span><span>Why do different chemicals emit light of different colour?</span><span>
</span>
Answer:
Protein Concentration is 2.82mg/L
Explanation:
According to Beer-Lambert's Law, Absorbance is directly proportional to the concentration.
However, the concentration of a solution can be determined from a calibration curve, in which Absorbance is plotted on the y-axis and the Concentration on the x-axis.
Plotting the best line, the equation of line is used
y = mx + c
where y is absorbance = 0.150
m is slope = 0.0163
x is concentration
c is intercept = 0.104
inserting the values from the question
y = mx + c
0.150 = 0.0163x + 0.104
0.0163x = 0.150 - 0.104
0.0163x = 0.046
Divide both sides by 0.0163
0.0163x/0.0163 = 0.046/0.0163
x = 2.82
Concentration of protein = 2.82 mg/L
It is the nimbostratus cloud.
