The heat generated when chemical, transport, or mechanical work is done by the organism is lost to the environment.
The the matter from one organism to the other is transferred via energy. The producer's produce and consumer's consume this energy according to 10% law. Most of the energy is lost (90% ) to the environment in form of heat.
Liquid 2 because the lower the density the more it floats and the higher the density the more it sinks. The order from top to bottom is liquid 2, liquid 3, liquid 1
