<span> These related to the polarities of the column and of the eluting solvent </span><span>When you run a column are
1) always start with a more non-polar solvent ratio and SLOWLY increase the polarity of the solvent.
1) Columns are generally made with silica which is highly polar, so compounds that are more polar in nature will interact with the silica more than those that are non-polar.
3) The first fractions to come out should be most non-polar, with each fraction collected after that being more and more polar.
hope this helps</span>
You can put it in a very strong magnetic field.
B the heart muscle gets too little oxygen
Helium is a gas composed of itself, were air is composed of Oxygen and Nitrogen making the air more dense than the helium making it float to find other gases
Two factors to change are Air pressure (due to the balloon moving ) and the helium making the balloon rise.
That's about all I can explain...hope this helps >.<