Question

You need to make 25 microliters of a 3M NaOh solution for a test reagent. Your laboratory routinely stocks 500 milliliters of a 10M NaOh solution. How would you prepare your solution? 


Answer 1

Answer:

0.0075 milliliters (7.5 microliters) will be taken from the stock solution and then diluted with 0.0249925 milliliters (24.9925 microliters) of water to make 0.025 milliliters (25 microliters) of NaOH solution.

Explanation:

This is a problem of dilution using the equation:

initial concentration x initial volume = final concentration x final volume.

The final volume to be prepared is 25 microliters.

The final concentration to be prepared is 3 M.

The initial volume to be taken is not known yet.

The initial concentration is 10 M.

Now, let's substitute these parameters into the the equation above.

10 x initial volume = 3 x 25

Initial volume = 3 x 25/10

     = 7.5 microliters

Note that: 1 microliter = 0.001 milliliters

Hence,

7.5 microliters = 0.0075 milliliters

This means that an initial volume of 0.0075 milliliters (7.5 microliters) will be taken from the stock solution. This amount will then be diluted with 0.0249925 milliliters (24.9925 microliters) of water to make 0.025 milliliters (25 microliters) of NaOH solution.