The purpose of using distilled water in the cuvette of the spectrophotometer is to calibrate the instrument. It is generally considered that distilled water shows zero absorbance and 100 % transmittance. So, to zero out the absorbance of compounds other than the analyte being determined, distilled water is used as a blank.
If the unknown being determined is prepared using ethanol as the base solution, the blank used must be ethanol. This is because absorbance if any from the solvent, ethanol must be zeroed out as when the measurement of the actual unknown is being made, the absorbance of the solvent does not interfere.
The path of energy flow from the sun to the humpback whale is as follows:
- Sun---> Plankton ---> Small fishes ---> Humpback whale.
<h3>What is energy?</h3>
Energy is the ability to do work.
The primary source of energy on the earth is the sun.
The energy from the sun is used by producers to produce food on which other organisms depend on.
The energy from the sun gets to the humpback whale through producers such as plankton.
The path of energy flow from the sun to the humpback whale is as follows:
- Sun---> Plankton ---> Small fishes ---> Humpback whale.
Learn more about energy flow at: brainly.com/question/21786633
Yes if you add an energy to an electron the electron will become excited, and it will jump to its highest level then go back down releasing energy