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Iron, water, sugar, salt, nitrogen gas, and oxygen gas
Holmes said to Watson "My dear doctor, this is a time for observation, not talk, because it was part of his method to observe closely and then to consider his observations. The correct answer is A.
Pure culture can be prepared from a mixture of cultures by using the streak plate method. This enables the bacterial cells to be diluted to obtain a pure culture after many-folds dilution.
Pure culture is the one in which only one species, strain or clone of bacteria are grown. The pure culture enables to scientists to study in detail about its anatomy, lifestyle and potential uses or harms, without the interference of any other microbe.
Streak-plate method is the most commonly used technique in microbiology labs to isolate a single type of bacteria. It is performed by creating the smears of bacteria in zig-zag motion to dilute its concentration.
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