Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.

The stock concentration of NaCl (5 M ) is as follows:
.

The stock concentration of Imidazole (1 M ) is as follows:
.

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer
Africa
Explanation:
Burning fossil fuels and deforestation is disturbingly heightened in Africa with almost no viable measure taking to control it's effect. No effective programs are presently in place to control excessive tree falling used for herbs, firewood, charcoal business and a whole lot. Below standard carbon rating PMS are in high circulation with fumes, carbon monoxide is full abundance in the atmosphere. Presently, all these unchecked activities have resulted into unexplainable excessive increase in temperatures, breeding neck twisting diseases, severe skin infections, dehydration and increase diabetes. Drought has eaten up landlocked cities in Nigeria and Central Africa. Infact farmers have lost count of the usual natural cycle of rainfall and the beginning of each farming season due to this essential evil of climate change
There is a bout 7 grams of protein in 1 ounce of meat, so 7*70=
Answer:
oxide of potassium or potassium oxide i believe.
Answer:
The correct answer is - option D. the boiling point of solution A will be lower than the boiling point of solution B
Explanation:
Colligative properties such as a decrease in the freezing point of the solution, increase in the boiling point of substance, decrease in Lowering of vapor pressure, and other properties depend upon the number of molecules only.
In the given solution the equal amount of two solutions are mixed that is 50 grams however due to the difference in the molecular mass so the atoms present in both solution A and B will be different. It is known that the number of atoms of a substance is inversely proportional to the molecular mass of the particular substance.
As it is given that Solution B has a low molecular mass which means it has a high number of atoms that means its boiling point will be higher than solution A.