How do you calculate mass using density and volume??

A 5.00-mL sample of blood was treated with trichloroacetic acid to precipitate proteins. After centrifugation, the resulting sol

Anarel [89]

Explanation:

According to the Beer-lambert' s law,

Absrobance = absrobptitvity × lenght × concentration

So, if we will plot a graph between absrobance and concentration then we will obtain a straight line

Hence, formula to calculate slope of the graph is as follows.

The slope of graph = $\frac{\text{difference in absrobance at two points}}{\text{difference in concentrations at those points}}$

The given data is as follows.

$A_{1}$ = 0.412 (first point)

$A_{2}$ = 0.642 (second point)

$C_{1}$ = 0.240 ppm (first point)

$C_{2}$ = 0.475 ppm (second point)

Hence, putting these values into the above formula we will calculate the value of slope as follows.

Slope = $\frac{\text{difference in absrobance at two points}}{\text{difference in concentrations at those points}}$

= $\frac{0.642 - 0.412}{0.475 - 0.240}$

= $\frac{0.23}{0.235}$

= 0.978

It is known that for the graph, line equation can be written as follows.

y = mx + c ........... (1)

where, C = intercept

m = slope

Hence, calculate the value of intercept as follows.

0.412 = $0.24 \times 0.978$ + c

c = 0.178

As it is given that the absorbance values 0.454 (the y-axis value). Therefore, putting this value into equation (1) we get the following.

y = mx + c

0.454 = $x \times 0.978$ + 0.178

0.276 = 0.978x

x = 0.282 ppm

Thus, we can conclude that the concentration of Pb in the given sample is 0.282 ppm.

3 0

3 years ago

Select True or False: A "gas" is a substance in which the molecules are separated on the average by distances that are large com

marta [7]

Answer:

True

Explanation:

The gaseous state is characterized in that the cohesion forces are usually null, in which the particles have their maximum mobility. The particles tend to occupy all the available volume, so their shape and volume are variable. The gaseous state is a dispersed state of matter, which means that the molecules are separated by distances much larger than the diameter of the gas molecules.

5 0

2 years ago

How many glucose molecules can be produced per second in a plant

Masja [62]

Since glycolysis of one glucose molecule generates two acetyl CoA molecules, the reactions in the glycolytic pathway and citric acid cycle produce six CO2 molecules, 10 NADH molecules, and two FADH2 molecules per glucose molecule

3 0

3 years ago

Which statement best explains how the conditions identified in Part A affect the availability of opal?

viktelen [127]

Answer:

C

Explanation:

5 0

3 years ago

A person breathes 2.6 L of air at -11 C into her lungs, where it is warmed to 37 C. What is its new Volume?

aalyn [17]

Answer:

3.076 L.

Explanation:

The pressure is constant as it is the atmospheric pressure.

According to Charles’ law; at constant pressure, the volume of a given quantity of a gas varies directly with its temperature.

∴ V₁T₂ = V₂T₁.

V₁ = 2.6 L & T₁ = -11 °C + 273 = 262 K.

V₂ = ??? L & T₂ = 37 °C + 273 = 310 K.

∴ V₂ = (V₁T₂) / T₁ = (2.6 L)(310 K) / (262 K) = 3.076 L.

5 0

3 years ago