Probably Fresh vegetables, it can rot out, that’d be my guess, it’s not canned
Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
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The reducing agent can approach the carbonyl face of camphor by forming a one carbon bridge (known as an exo attack) or a two carbon bridge (termed endo).
The two resultant stereoisomers are known as isoborneol and borneol (from exo attack) (from endo attack). Gas chromatography (GC) analysis may be used to calculate the ratio of each isomeric alcohol in the mixture. Unfortunately, IR analysis does not permit this.
The stereochemistry of the reaction is regulated in stiff cyclic compounds like camphor and norcamphor by protecting one side of the carbonyl group from the reagent's assault. The hydrogen atom is added to the endo side, creating the exo alcohol isoborneol, while the methyl groups on the one-carbon bridge of camphor screen the approach of the hydride from the "top" or exo side of the two-carbon bridge. You will be asked to guess the main isomeric alcohol created by the norcamphor hydride reduction later in the lab report.
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Number of significant digits is 3.
Answer:Free radical mono-halogenation of an alkane is typically conducted using bromine versus chlorine because the bromine radical is less reactive and therefore more selective.
Explanation: Halogenation occurs when a halogen replaces one or more hydrogen atoms in an organic compound ie chlorine or bromine with the reactivity of the halogens decreasing in the order of F2 > Cl2 > Br2 > I2
Since fluorine reacts explosively making it is difficult to control, and iodine is unreactive. Free radical mono-halogenation of an alkane is typically conducted using bromine versus chlorine with Chlorination ie chlorine radical being more reactive and not selective and the Bromination of alkanes ie bromine radical occurring similarly but slower and less reactive but more selective which is due to the fact that a bromine atom is less reactive in the hydrogen abstraction than a chlorine atom evidence in the higher bond energy of H-Cl than H-Br.
