Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.
The answer is A because if you place the first two numbers 1 and 3
Au=2
S=3
H=6
It has to equal to the product so then you put in the last two numbers 2 and 3
Au=2
S=3
H=6
25.13(5512)
4568%of the time is 56789
5678
4333
Answer : The amount of energy needed to raise one gram of a substance one degree Celsius is a characteristic property known as, Specific heat capacity.
Explanation:
Heat capacity : It is defined as the heat required to raise the temperature by one degree.
Specific heat capacity : It is defined as the amount of heat required by one gram of a substance to raise its temperature by one degree Celsius.
The heat required to change the temperature of a substance is related to heat capacity of the substance by the expression as :
where,
C = specific heat capacity
m = mass of a substance
q = heat required
= change in temperature of substance
Hence, the amount of energy needed to raise one gram of a substance one degree Celsius is a characteristic property known as, Specific heat capacity.
