Answer:
Taq polymerase is a thermostable which states that it can even work at higher temperature. The main function of using this enzyme is that it is used to amplify the DNA which will help in producing ample amount of DNA sample.
The enzyme activity is temperature dependent. The denaturation and annealing steps of the PCR occurs at very high temperature.
Any other enzyme used at such an high temperature would have decreased the enzyme activity and the procedure would not be completed.
The solution is buffered with a weak acid and it's conjugate base. Since NaOH is a base, you can use a weak acid as it's buffer.
Answer:
Energy can neither be created nor destroyed, but it does change its form. And not all forms of energy are usable and it gets dissipated as heat energy and sound energy. The energy that is wasted cannot be recovered.
Was there supposed to be a picture?
Answer:
Mass = 5.92 g
Explanation:
Given data:
Volume of O₂ = 4.15 mol
Temperature and pressure = standard
Mass in gram = ?
Solution:
The given problem will be solve by using general gas equation,
PV = nRT
P= Pressure
V = volume
n = number of moles
R = general gas constant = 0.0821 atm.L/ mol.K
T = temperature in kelvin
By putting values,
1 atm × 4.15L = n ×0.0821 atm.L /mol.K × 273.15 k
4.15 atm.L = n ×22.43 atm.L /mol
n = 4.15 atm.L / 22.43 atm.L /mol
n = 0.185 mol
Mass in gram:
Mas = number of moles × molar mass
Mass = 0.185 mol ×32g/mol
Mass = 5.92 g
