Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Surface tension is the result of water molecules pulling inward with a strong attractive force.
The attractive force brings the molecules on the surface of the water close together.
The attractive force causes the surface of the water to be drawn toward the water molecules beneath the surface. <span>
Since there are no water molecules above the surface, there are uneven forces. This causes surface tension, allowing the coin to float on the water’s surface.</span>
<span>If thermal energy (heat) must be added to a chemical reaction in order for the reaction to take place, the reaction is endothermic. </span>
POH = - log [ OH⁻ ]
pOH = - log [ 1 x 10⁻⁹ ]
pOH = 9
Answer C
hope this helps!
Roughly 39 grams, give or take 1 gram
