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Veseljchak [2.6K]
3 years ago
15

Two DNA sequences, each 48 kb in size, are treated with the same restriction enzyme. There is only one site for this restriction

enzyme on each of these sequences. Digests are separated by gel electrophoresis. Sequence A shows ONE band that is 48 kb in size. Sequence B shows TWO bands, one 30 kb and the other 18 kb in size What can you conclude from these data?
A. Sequence A is linear, sequence B is circular
B. Both sequences are linear. >X
C. They are identical sequences
D. Sequence A is circular, sequence B is linear
Chemistry
1 answer:
morpeh [17]3 years ago
7 0

Answer:

Sequence A is circular, sequence B is linear.

Explanation:

Restriction enzymes is also known as the molecular scissors that cuts the specific DNA fragments. Two main types of restriction enzymes are restriction exonuclease and restriction endonuclease.

The DNA fragments that are digested by the restriction endonuclease can be used for the preparation of the maps. The circular DNA ganaretes the equal number of fragments as it has the restriction site and linear DNA produce one extra fragments.

Thus, the correct answer is option (D).

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If a compound has a composition of 82% nitrogen and 18% hydrogen, what is the empirical formula for this compound
Damm [24]

Answer: The empirical formula for the given compound is NH_3

Explanation : Given,

Percentage of H = 18 %

Percentage of N = 82 %

Let the mass of compound be 100 g. So, percentages given are taken as mass.

Mass of H = 18 g

Mass of N = 82 g

To formulate the empirical formula, we need to follow some steps:

Step 1: Converting the given masses into moles.

Moles of Hydrogen = \frac{\text{Given mass of Hydrogen}}{\text{Molar mass of Hydrogen}}=\frac{18g}{1g/mole}=18moles

Moles of Nitrogen = \frac{\text{Given mass of nitrogen}}{\text{Molar mass of nitrogen}}=\frac{82g}{14g/mole}=5.8moles

Step 2: Calculating the mole ratio of the given elements.

For the mole ratio, we divide each value of the moles by the smallest number of moles calculated which is 5.8 moles.

For Hydrogen  = \frac{18}{5.8}=3.10\approx 3

For Nitrogen = \frac{5.8}{5.8}=1

Step 3: Taking the mole ratio as their subscripts.

The ratio of H : N = 3 : 1

Hence, the empirical formula for the given compound is NH_3

3 0
3 years ago
An exothermic reaction
Irina-Kira [14]

Answer:

the energy of the products of the cause of reactants.It's most common with endothermic reaction but vice-versa.

Explanation:

5 0
3 years ago
Read 2 more answers
A sample of P2Cl5 contains 179 g of phosphorous, how many grams of chlorine are present? Please show step by step thx!
natima [27]

Answer:

About 512 g.

Explanation:

We are given a sample of P₂Cl₅ that contains 179 grams of phosphorus, and we want to determine the grams of chlroine that is present.

Thus, we can convert from grams of phosphorus to moles of phosphorus, moles of phosphorus to moles of chlorine, and moles of chlorine to grams of chlorine.

From the formula, there are two moles of P for every five moles of Cl. The molecular weights of P and Cl are 30.97 g/mol and 35.45 g/mol, respectively. Hence:

\displaystyle 179\text{ g P} \cdot \frac{1\text{ mol P}}{30.97\text{ g P}} \cdot \frac{5\text{ mol Cl}}{2\text{ mol P}} \cdot \frac{35.45\text{ g Cl}}{1\text{ mol Cl}} = 512\text{ g Cl}

In conclusion, there is about 512 grams of chlorine present in the sample.

Alternatively, we can mass percentages. The mass percent of phosphorus in P₂Cl₅ is:


\displaystyle \% \text{P} = \frac{2(30.97)}{5(35.45) + 2(30.97)} = 25.90\%

Because there are 179 grams of phosphorus, the total amount of sample present is:


\displaystyle \begin{aligned} 25.90\% \cdot  m_T  & = 179\text{ g P} \\ \\ m_T & = 691.1 \text{ g}\end{aligned}

Therefore, the amount of chlorine present is 691.1 g - 179 g, or about 512 g, in agreement with our above answer.

8 0
3 years ago
How many miles of Fe2+ ions and MnO4- ions were titrated in each part 1 trial
Fudgin [204]

Answer:

In 1000 ml there is 0.10 moles of Fe 2+

Therefore, in 10 ml there is (0.1/1000)*10= 0.001 mol of Fe2+

mole ratio for rxn Fe2+ : MnO4- is

1 : 2

therefore if 0.001 moles of Fe2+ react then 0.001*2 =0.002 moles of MnO4- react with Fe2+

hence, molarity of MnO4- = (mol*vol)/1000

= 0.002*10.75/1000= 2.15*10-5M

Explanation:

Hope this helps

5 0
4 years ago
The octane rating of gasoline is a measure of the resistance to premature ignition, also known as engine knock. It is based on c
SIZIF [17.4K]

Answer: From the attached document

1. Complete structure

2. Skeletal structure

5 0
3 years ago
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