Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
answer: focus or hypocenter
enplanation: when energy release at the focus, seismic waves travel outward from that point in all directions.
Answer:
Abiotic objects
Ex. bed, table, lamp, chair, blanket, etc.
Abiotic means Non-living.
Answer:
Group 1
Explanation: because the compound has a formula of M2O , the number of valence electrons of M should be 1.
Answer:
531–1532 – Pizarro's third voyage to Peru. Spaniards form a bond with the Natives (Huancas, Chankas, Cañaris and Chachapoyas) who were under the oppression of the Inca Empire, and Pizarro includes them among his troops to face the Incas. Atahualpa is captured by Spanish.
Explanation: Is this right ?
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