Mass of aspirin = 0.025 g
Molar mass of C9H8O4 is 180.1583 g/mol
moles of aspirin = .025g / 180.1583 g/mol = 0.000138767 moles
volume solution = .250 L
molarity of the solution = 0.000138767 moles / .250L =5.551 x 10 ^-04 Moles / liter
for aspirin i = Vant'Hoff factor = 1 particle in solution
T = 25 + 273 =298 K
osmotic pressure = M x R x T x i =
5.551 x 10 ^-04 mole L -1 x 0.08206 L atm K−1 mol−1 x 298 K x 1 = 0.0136 atmospheres
Answer:
D. pUC-chloramphenicol(minus)
Explanation:
It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.