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Evgesh-ka [11]
3 years ago
8

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at th

e active site; this enzyme-inhibitor bond is not cleaved by the enzyme. This is an example of what kind of inhibition? A) Irreversible B) Competitive C) Non-competitive D) Mixed E) pH inhibition
Chemistry
1 answer:
dolphi86 [110]3 years ago
5 0

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site, this enzyme-inhibitor bond is not cleaved by the enzyme. This is an example of an Irreversible kind of inhibition.

<u>Explanation:</u>

  •    Cell Lysates are prepared by using Phenyl-methane-sulfonyl-fluoride (PMSF). This PMSF is an enzyme inhibitor that inactivates serine proteases.
  •     It inactivate the serine proteases by attaching with the catalytic serine, Which was in an active state.
  •    The PMSF bond was not separated by the enzyme. So this action cannot be repeated. Hence it is an irreversible kind of inhibition.
  •     Handling of PMSF should be done in a fume hood and while handling it we should wear gloves.
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Why do the metals Co, Rh, and Lr form octahedral complexes (rather than tetrahedral or square planar complexes)? Hint: Look at t
algol13

Explanation:

Octahedral complexes will be favoured over tetrahedral ones because:

It is more favourable to form six bonds rather than four

The crystal field stabilisation energy is usually greater for octahedral than tetrahedral complexes.

The transition metals Co, Rh, Lr are in group 9 of d block and they have 3d, 4d, and 5d orbitals respectively

6 0
3 years ago
A mixture of hydrogen (2.02 g) and chlorine (35.90 g) in a container at 300 K has a total gas pressure of 748 mm Hg. What is the
Llana [10]

The partial atmospheric pressure (atm) of hydrogen in the mixture is 0.59 atm.

<h3>How do we calculate the partial pressure of gas?</h3>

Partial pressure of particular gas will be calculated as:

p = nP, where

  • P = total pressure = 748 mmHg
  • n is the mole fraction which can be calculated as:
  • n = moles of gas / total moles of gas

Moles will be calculated as:

  • n = W/M, where
  • W = given mass
  • M = molar mass

Moles of Hydrogen gas = 2.02g / 2.014g/mol = 1 mole

Moles of Chlorine gas = 35.90g / 70.9g/mol = 0.5 mole

Mole fraction of hydrogen = 1 / (1+0.5) = 0.6

Partial pressure of hydrogen = (0.6)(748) = 448.8 mmHg = 0.59 atm

Hence, required partial atmospheric pressure of hydrogen is 0.59 atm.

To know more about partial pressure, visit the below link:
brainly.com/question/15302032

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3 0
2 years ago
If a 1.00 mL sample of the reaction mixture for the equilibrium constant experiment required 32.40 mL of 0.258 M NaOH to titrate
andrey2020 [161]

Answer:

The concentration of acetic acid is 8.36 M

Explanation:

Step 1: Data given

Volume of acetic acid = 1.00 mL = 0.001 L

Volume of NaOH = 32.40 mL = 0.03240 L

Molarity of NaOH = 0.258 M

Step 2: The balanced equation

CH3COOH + NaOH → CH3COONa + H2O

Step 3: Calculate the concentration of the acetic acid

b*Ca*Va = a*Cb*Vb

⇒with b = the coefficient of NaOH = 1

⇒with Ca = the concentration of CH3COOH = TO BE DETERMINED

⇒with Va = the volume of CH3COOH = 1.00 mL = 0.001L

⇒with a = the coefficient of CH3COOH = 1

⇒with Cb = the concentration of NaOH = 0.258 M

⇒with Vb = the volume of NaOH = 32.40 mL = 0.03240 L

Ca * 0.001 L = 0.258 * 0.03240

Ca = 8.36 M

The concentration of acetic acid is 8.36 M

6 0
3 years ago
A beaker containing 6.32 moles of PBr3, contains___
NISA [10]

Answer:

3.8 x 10²⁴molecules

Explanation:

Given parameters:

Number of moles  = 6.32moles

Unknown:

Number of molecules  = ?

Solution:

The number of moles can be used to derive the number of molecules found within a substance.

Now,

       1 mole of substance contains 6.02 x 10²³ molecules

     6.32 mole of PBr₃ will contain 6.32 x 6.02 x 10²³ = 3.8 x 10²⁴molecules

8 0
3 years ago
Use the drop-down menus to complete each statement.
aleksley [76]

<u>Explanation:</u>

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<u>2. Have a rocky composition:</u>

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<u>3. Revolve quickly around the Sun: </u>

  • Mercury is the quickest planet, which rushes around the sun at 47.87 km/s. And it revolves around the sun quickly.

<u> 4. Rotate quickly on their axes: </u>

  • The giant gas planets like Jupiter, Saturn, etc... spin more quickly on their axes than the other planets

6 0
3 years ago
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