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Marizza181 [45]
3 years ago
5

What is the most efficient way to make up a hepes buffer at ph 8.5?

Chemistry
1 answer:
ruslelena [56]3 years ago
4 0

pka value of HEPES is 7.5.

A proper amount of HEPES is mixed with distilled water (sterilized) to avoid any contamination. After that, check the pH with the help of pH scale or pH meter.

First, take the basic form of HEPES and then add hydrochloric acid drop by drop to bring down the pH at 8.5.

When pH reaches 8.5; the buffer solution is filtered and placed in the  glass container and then covered by aluminium foil.

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Molarity of 2%W/V of NaOH is
TiliK225 [7]
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How many protons are found in an atom of platinum (pt)?
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Must show units and how they cancelli 1.) 175 km to um 3.) 385 nm to dm 5.) 492 um tom 7.) 52 x 103 dm to mm 9.) 321x 1035 mm to
morpeh [17]

Explanation:

1.) 175 km to μm

1 km=10^9 \mu m

175 km=175\times 10^9\mu m=1.75\times 10^{11} \mu m

3.) 385 nm to dm

1 nm=10^{-8} dm

385 nm=385\times 10^{-8} dm=3.85\times 10^{-6} dm

5.) 492 μm  to m

1 μm =  10^{-6} m

492 \μm=492\times 10^{-6} m=4.92\times 10^{-4} m

7.) 52\times 10^3 dm to mm

1 dm = 100 mm

52\times 10^3 dm=52\times 10^3\times 100 mm=5.2\times 10^{6}dm

9.) 321\times 10^{35} mm to km

1 mm = 10^{-6} km

321\times 10^{35} mm=321\times 10^{35}\times 10^{-6} km=3.21\times 10^{31} km

11.) 456\times 10^3 m to km

m = 0.001 km

456\times 10^3m =456\times 10^3 m\times 0.001 km=456 km

13.) 422\times 10^3 m to nm

1 m = 10^{9} nm

422\times 10^3 m=422\times 10^3\times 10^{9} nm=4.22\times 10^{14} nm

15.) 4.87\times 10^{30} m to pm

1 m = 10^{12} pm

4.87\times 10^{30} m=4.87\times 10^{30}\times 10^{12} pm=4.82\times 10^{42} pm

17.) 5.26\times 10^3 m to um

1 m =  10^{6} \mu m

5.26\times 10^3 m=5.26\times 10^3\times 10^6 \mu m=5.26\times 10^{9} \mu m

19.) 1.25\times 10^{35}m to Mm

1 m =  10^{-6} Mm

1.25\times 10^{35} m=1.25\times 10^{35}\times 10^{-6} Mm=1.25\times 10^{-29} Mm

21.) 4.22\times 10^3 Tm to nm

1 Tm = 10^{21} nm

4.22\times 10^3 Tm=4.22\times 10^3\times 10^{21} nm=4.22\times 10^{24} nm

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