What is the most efficient way to make up a hepes buffer at ph 8.5?
1 answer:
value of HEPES is 7.5.
A proper amount of HEPES is mixed with distilled water (sterilized) to avoid any contamination. After that, check the pH with the help of pH scale or pH meter.
First, take the basic form of HEPES and then add hydrochloric acid drop by drop to bring down the pH at 8.5.
When pH reaches 8.5; the buffer solution is filtered and placed in the glass container and then covered by aluminium foil.
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Answer : The metal used was iron (the specific heat capacity is ).
Explanation :
In this problem we assumed that heat given by the hot body is equal to the heat taken by the cold body.
where,
= specific heat of metal = ?
= specific heat of water =
= mass of metal = 47.1 g
= mass of water = 120 g
= final temperature of water =
= initial temperature of metal =
= initial temperature of water =
Now put all the given values in the above formula, we get
Form the value of specific heat of metal, we conclude that the metal used in this was iron.
Therefore, the metal used was iron (the specific heat capacity is ).