What is the most efficient way to make up a hepes buffer at ph 8.5?
1 answer:
value of HEPES is 7.5.
A proper amount of HEPES is mixed with distilled water (sterilized) to avoid any contamination. After that, check the pH with the help of pH scale or pH meter.
First, take the basic form of HEPES and then add hydrochloric acid drop by drop to bring down the pH at 8.5.
When pH reaches 8.5; the buffer solution is filtered and placed in the glass container and then covered by aluminium foil.
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Answer: There are now 2.07 moles of gas in the flask.
Explanation:
P= Pressure of the gas = 697 mmHg = 0.92 atm (760 mmHg= 1 atm)
V= Volume of gas = volume of container = ?
n = number of moles = 1.9
T = Temperature of the gas = 21°C=(21+273)K= 294 K (0°C = 273 K)
R= Value of gas constant = 0.0821 Latm\K mol
When more gas is added to the flask. The new pressure is 775 mm Hg and the temperature is now 26 °C, but the volume remains same.Thus again using ideal gas equation to find number of moles.
P= Pressure of the gas = 775 mmHg = 1.02 atm (760 mmHg= 1 atm)
V= Volume of gas = volume of container = 49.8 L
n = number of moles = ?
T = Temperature of the gas = 26°C=(26+273)K= 299 K (0°C = 273 K)
R= Value of gas constant = 0.0821 Latm\K mol
Thus the now the container contains 2.07 moles.