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Marizza181 [45]
3 years ago
5

What is the most efficient way to make up a hepes buffer at ph 8.5?

Chemistry
1 answer:
ruslelena [56]3 years ago
4 0

pka value of HEPES is 7.5.

A proper amount of HEPES is mixed with distilled water (sterilized) to avoid any contamination. After that, check the pH with the help of pH scale or pH meter.

First, take the basic form of HEPES and then add hydrochloric acid drop by drop to bring down the pH at 8.5.

When pH reaches 8.5; the buffer solution is filtered and placed in the  glass container and then covered by aluminium foil.

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When 33 g of CaO and 10 g of H2O react, how many grams of calcium hydroxide would you expect to be produced* Explain your answer
MrMuchimi

Answer:

41.44 g

Explanation:

First of all, we must put down the equation of the reaction;

CaO + H2O ----->Ca(OH)2

Number of moles of CaO = 33g/56 g/mol = 0.59 moles

Number of moles of H20 = 10g/18 g/mol = 0.56 moles

Since the reaction is in 1:1 mole ration, H2O is the limiting reactant

Hence;

mass of Ca(OH)2 produced = 0.56 moles * 74 g/mol = 41.44 g

7 0
3 years ago
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Which of the following statements is (are) true about enzyme-catalyzed reactions? a. The reaction is faster than the same reacti
Brums [2.3K]

Answer : The correct option is A.

Explanation :

Enzyme-catalyzed reaction :

Enzyme act as a biological catalyst and the role of catalyst is to increase the rate of chemical reaction by lowering the activation energy.

Most of the chemical reactions are slow in the absence of enzyme but in the presence of enzyme, the reaction become faster. That means the Enzyme accelerate the rate of reaction.

Therefore, the correct answer is the reaction is faster than the same reaction in the absence of the enzyme.

5 0
3 years ago
Convert 15.6 g Na2Cr2O7 to moles of Na2Cr2O7.
Leviafan [203]

Answer:

261.96754

Explanation:

3 0
3 years ago
i have a crossword thing that i got from school and one of the clues is: If there are two equal and opposite forces on something
cluponka [151]
I would assume the answer is called attractive
6 0
2 years ago
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Nitrogen effuses through a pinhole 1.7 times as fast as another gaseous element under the same conditions. Estimate the other el
marysya [2.9K]

Answer:

80.92, Krypton

Explanation:

<u>What is effusion?</u>

• It is a process where gas escapes through a pinhole (a very small hole) into a region of low pressure or vacuum

<u>Graham's law of effusion of </u><u>gas</u>

• states that at a given constant temperature and pressure, the rate of effusion of gases is inversely proportional to the square root of their molar masses

\boxed{ \frac{Rate_1}{Rate_2} =  \sqrt{ \frac{M_2}{M_1} } }

<u>Calculations</u>

Nitrogen exist as N₂ at room temperature, thus its molar mass is 2(14)= 28.

Let the rate and molar mass of unknown gas be Rate₂ and M₂ respectively.

Since N₂ effuses 1.7 times as fast as the unknown gas,

Rate₁= 1.7(Rate₂)

\frac{Rate_1}{Rate_2} = 1.7

1. 7 =  \sqrt{ \frac{M_2}{28} }

Square both sides:

2.89  = \frac{M_2}{28}

Multiply both sides by 28:

2.89(28)= M₂

M₂= 80.92

<u>Identity of </u><u>gas</u>

The molar mass of 80.92 lies between Bromine and Krypton. However since Bromine exist as Br₂, the value of it's molar mass would be 159.8 instead. Hence, Bromine is eliminated.

If the gas is a diatomic element, the atomic weight is 80.92 ÷2= 40.46. Thus, we are now considering if Argon could be its identity. However, Argon is a noble gas and will not exist as a diatomic element. Argon is therefore eliminated too.

Thus based on the above reasoning, its probable identity is Krypton.

7 0
2 years ago
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