Question

How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 0.2 mL? 1 microliter is 10-6 L or 10-3 mL.

Answer 1

Answer:

The required volume of the original sample required is 2 micro liter

Explanation:

assuming the original sample concentration is 1N

after  final dilution of 10-2 solution concentration becomes 0.01 N

normality of original sample  = 1 N

normality  of final solution = 0.01 N

volume of  original sample= ?

volume of   final solution  = 0.2 mL

Considering thef formula below :

N1V1 = N2V2

V1 = (N2V2)/N1

= (0.01*0.2)/1

= 0.002 mL

1 milli liter = 1000 micro liter

0.002 mL = 2 micro liter

The original sample required is 2 micro liter