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baherus [9]
3 years ago
12

The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1, pK2, and pKR of the amino acid arg

inine are 1.8, 9.0, and 12.5, respectively. A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine. What pH is likely to work best for this separation?
Chemistry
1 answer:
almond37 [142]3 years ago
5 0

Answer:

pH 9,8 is likely to work best for this separation

Explanation:

Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).

For lysine, PI is:

pH = \frac{1}{2} (9,1+10,5) = 9,8

For arginine:

pH = \frac{1}{2} (9,0+12,5) = 10,75

At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.

Thus, <em>pH 9,8 is likely to work best for this separation</em>

<em></em>

I hope it helps!

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An oxide of nitrogen contains 30.45 mass % N.
N76 [4]

An oxide of nitrogen contains 30.45 mass % N, if the molar mass is 90± 5 g/mol the molecular formula is N₂O₄.

<h3>What is molar mass?</h3>

The molar mass of a chemical compound is determined by dividing its mass by the quantity of that compound, expressed as the number of moles in the sample, measured in moles. A substance's molar mass is one of its properties. The compound's molar mass is an average over numerous samples, which frequently have different masses because of isotopes.

<h3>How to find the molecular formula?</h3>

The whole-number multiple is defined as follows.

Whole-number multiple = \frac{molar mass (g/mol)}{empirical formula mass (g/mol)}

The empirical formula mass is shown below.

Mw of empirical formula = Mw of N+ 2 x (Mw of O)

                                      = 14.01 g/mol + 2 x (16.00 g/mol)

                                      = 46.01 g/mol

With the given molar mass or the molecular formula mass, we can get the whole-number multiple for the compound.

Whole-number multiple = \frac{90 g/mol }{46.01 g/mol} ≈ 2

Multiplying the subscripts of NO2 by 2, the molecular formula is N(1x2)O(2x2)= N2O4.

To learn more about molar mass visit:

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5 0
2 years ago
What type of cryptography uses two keys instead of just one, generating both a private and a public key?
Alexxx [7]

Answer:

It is known as asymmetric key cryptography it is also called public key cryptography.

Explanation:

Asymmetric key cryptography method makes use of two keys.One is used for encryption and the second one for decryption. The public key serves to encrypt plain text or verify a digital signature, while the private key is used to decrypt or decipher the encrypted text or to create a digital signature.

3 0
3 years ago
(-5)+(+7)-(-4) + (+12)<br>A 8<br>B 10<br>C 18<br>d 20<br>​
Vladimir79 [104]

Answer:

C-18

Explanation:

Step one follow order of operations

Add and subtract from left to right(-5)+(7)=2-(-4)+(12)

STEP 2

Apply negative Rule -(-4)=+4=2+4+(12)

then add 2+4+12=18

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3 years ago
Acetone, the solvent in nail polish remover, has a density of 0.761 g/ml What is the volume of 25.0g of acetone
Jlenok [28]

Answer: 100.0g

Explanation:

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1 year ago
How many moles of gas would occupy 22.4 L at 273K in one arm?
andrew-mc [135]

Answer:

1 mol

Explanation:

Using the general gas law equation as follows:

PV = nRT

Where;

P = pressure (atm)

V = volume (L)

n = number of moles (mol)

R = gas law constant (0.0821 Latm/molK)

T = temperature (K)

According to the provided information in the question;

V = 22.4L

T = 273K

P = 1 atm

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n = ?

Using PV = nRT

n = PV/RT

n = (1 × 22.4) ÷ (0.0821 × 273)

n = 22.4 ÷ 22.4

n = 1mol

4 0
2 years ago
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