The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The pK1, pK2, and pKR of the amino acid arg
1 answer:
Answer:
pH 9,8 is likely to work best for this separation
Explanation:
Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).
For lysine, PI is:
9,8
For arginine:
10,75
At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.
Thus, <em>pH 9,8 is likely to work best for this separation</em>
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Answer:
1) Fe = 69.9%
O = 31.1%
2) H = 5.19%
O = 16.5%
N = 28.9%
C = 49.5%
Explanation:
One easy way to do percent compositions is to assume you have 100g of a substance.
1) Lets say we have 100g of Fe2O3.
The total molar mass would be:
The molar mass of the Fe2 alone is:
Thus, the grams of Fe2(out of a 100) could be calculated by multiplying 100g * the molar mass ratio of Fe2 to the whole:
Which is approximately 69.9%.
We can find the amount of O3 by simply subtracting, as the rest of the compound is made of O3. Thus, the % composition of O3 is 31.1%
You can then do this same process to the next question, getting us the following:
H = 5.19%
O = 16.5%
N = 28.9%
C = 49.5%