Question

Genetic engineers must modify eukaryotic genes so that they can be expressed in bacterial host cells. The DNA from a eukaryotic gene cannot be placed directly into the bacteria but is first transcribed to mRNA and then reverse-transcribed back to cDNA. Explain why this is necessary.

Answer 1

Answer:

Bacterial genes contain no introns, so the cells, which lack splicing machinery, cannot express eukaryotic genes containing introns and exons. Mature eukaryotic mRNA, which has already been spliced, contains only exons, which, after they have been converted back to DNA, can be transcribed and translated by the bacteria.

Explanation: