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Olegator [25]
3 years ago
5

The suggested procedure for spotting a sample on a thin‑layer chromatography (TLC) plate involves transferring a droplet of conc

entrated solution from a capillary onto the plate, allowing it to dry, then repeating the process over the existing spot. Why is it advised to spot multiple times? to facilitate visualization by having a higher compound concentration to prevent the solvent in the developing chamber from evaporating to fill the capillary almost instantly by capillary action to achieve a high concentration of sample in a large spot
Chemistry
1 answer:
Mrrafil [7]3 years ago
6 0

Answer:

Correct is: to facilitate visualization by having a higher compound concentration

Explanation:

It is advised to spot multiple times to have a higher compound concentration (more compound in a small spot). If the spot is too big, the compound will disperse and run unevenly.

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