Answer:
pH 9,8 is likely to work best for this separation
Explanation:
Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).
For lysine, PI is:
9,8
For arginine:
10,75
At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.
Thus, <em>pH 9,8 is likely to work best for this separation</em>
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I hope it helps!
12.8 mole of CO2 from the combustion
3.65 X 10 to the power of 8
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The equation for the reaction is:
C₄H₈O₂ + C₂H₅OH = C₆H₁₂O₂ + H₂O
Now you see that the number of the moles of butanoic acid
and etyl butyrate is equal in
the reaction. That means;
number of moles of C₄H₈O₂ = number of moles of C₆H₁₂O₂
mass of C₄H₈O₂/ Molar mass of C₄H₈O₂ = mass of C₆H₁₂O₂/ molar mass of C₆H₁₂O₂
mass of C₆H₁₂O₂ = molar mass of C₆H₁₂O₂ x mass of C₄H₈O₂/ Molar mass of C₄H₈O₂
Now, assuming <span>100% yield, the mass
of ethyl butyrate produced is: </span>
<span>= 7.45/88.11 x 116.16</span>
<span>=9.82g</span>
<span>Thus, the theoretical yield of ethyl butyrate is 9.82g.</span>