Question

A protein sample has been treated differently before being loaded onto the gel for SDS-PAGE. The first aliquot of the sample is treated with SDS. The second aliquot of the sample is treated with SDS and a reducing agent. If there is no difference in the number of bands when comparing the results of the two experiments, the results most likely indicate _________.

Answer 1

Answer:

$\boxed{\text{There are no disulfide bonds in the protein}}$

Explanation:

The most easily reduced groups in a protein are disulfide bonds, RS-SH.

$\text{If there is no difference in results with and without a reducing agent,}\\\text{this indicates that \boxed{\textbf{there are no disulfide bonds in the protein.}}}$