Question

Select the statement that is true for why is a blank measured before the sample. Select one: a. A blank is measured to remove the signal caused by the cuvette walls and solvent. b. A blank is measured to calibrate the spectrophotometer's light source. c. There is no reason to measure a blank before the sample. d. A blank is measured to clean the spectrophotometer.

Answer 1

Answer:

Option A

A blank is measured to remove the signal caused by the cuvette walls and solvent

Explanation:

Spectrophotometer is an instrument for measuring the absorbance or transmittance of the liquid in terms of wave function. The blank solutions is used for calibration of spectrophotometer while the cuvette is the container where the liquid sample is taken in the spectrophotometer. The absorbance of blank solution is known as zero hence the name blank solution. Normally, a blank is is measured to eliminate signal caused by cuvette walls on solvent and results into the formation of solute peaks in the spectra.