What's wrong with this setup is the substrate on which you have positioned
the drop is "dirty and unclean" meaning it is not being dampened by
the solution. This action can be corrected by comprehensively cleaning the
substrate where the drop will be positioned.
<u>Answer:</u> The pH of the buffer is 5.25
<u>Explanation:</u>
Let the volume of buffer solution be V
We know that:

To calculate the pH of acidic buffer, we use the equation given by Henderson Hasselbalch:
![pH=pK_a+\log(\frac{[\text{conjugate base}]}{[acid]})](https://tex.z-dn.net/?f=pH%3DpK_a%2B%5Clog%28%5Cfrac%7B%5B%5Ctext%7Bconjugate%20base%7D%5D%7D%7B%5Bacid%5D%7D%29)
We are given:
= negative logarithm of acid dissociation constant of weak acid = 4.90
![[\text{conjugate base}]=\frac{2.25}{V}](https://tex.z-dn.net/?f=%5B%5Ctext%7Bconjugate%20base%7D%5D%3D%5Cfrac%7B2.25%7D%7BV%7D)
![[acid]=\frac{1.00}{V}](https://tex.z-dn.net/?f=%5Bacid%5D%3D%5Cfrac%7B1.00%7D%7BV%7D)
pH = ?
Putting values in above equation, we get:

Hence, the pH of the buffer is 5.25
Acid-base indicator changes color based on pH.
drop some in a solution n watch the color changes. different indicators show different colors at different pH. they usually have standard colors for comparison.
Time, Ice size, water temp